Leukocyte Elastase Release during Blood Coagulation

Leukocyte elastase release during blood coagulation. A potential mechanism for activation of the alternative fibrinolytic pathway.

Immunological detection of elastase, an enzyme present within leukocyte granules, has been used as a marker for polymorphonuclear leukocyte activation. Polymorphonuclear leukocytes contained 4.6 mug/10(7) cells, whereas erythrocytes, mononuclear cells, and platelets contained <1% of this level. In plasma that was separated from blood cells after 1 h at 22 degrees C, the mean level of elastase-r...

Human plasma kallikrein releases neutrophil elastase during blood coagulation.

Elastase is released from human neutrophils during the early events of blood coagulation. Human plasma kallikrein has been shown to stimulate neutrophil chemotaxis, aggregation, and oxygen consumption. Therefore, the ability of kallikrein to release neutrophil elastase was investigated. Neutrophils were isolated by dextran sedimentation, and elastase release was measured by both an enzyme-linke...

Determination of leukocyte elastase-inhibitor complexes and leukocyte neutral proteinase inhibitor by enzyme immunoassays. Leukocyte elastase-inhibitor complexes in porcine blood, III.

Sensitive enzyme immunoassays for the determination of total leukocyte neutral proteinase inhibitor and polymorphonuclear elastase-leukocyte neutral proteinase inhibitor complexes are described. The usable ranges of the standard curves were from 80 ng/l to 10 micrograms/l. The relative intra-assay coefficients of variation of the tests were between 2 and 4%, and the inter-assay coefficients of ...

[antihemophilic Globulin Consumption during Blood Coagulation].

Alpha - 1 - Antitrypsin - Human Leukocyte Elastase Complexes in Blood : Quantification by an Enzyme - Linked Differential Antibody

An enzyme-linked immunosorbent assay (ELISA) has been developed for the quantification of cr,-antitrypsin-human leukocyte elastase (a,AT-E) complexes. In the ELISA, the a,AT-E complex is bound to a surface by rabbit antileukocyte elastase antibody, and the inhibitor-proteinase cornplex is quantified by a second antibody, rabbit anti-a1antitrypsin F(ab’)2. labeled with alkaline phosphatase. a1AT...